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KMID : 0375319920140020259
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Journal of Clinical Pathology and Quality Control
1992 Volume.14 No. 2 p.259 ~ p.268
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Comparison of the Growth of Anaerobic Bacteria in Various Thioglycollate Media Used for Blood Culture
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Kim Mi-Hyang
Lee Kyung-Won
Chong Yun-Sop
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Abstract
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Depending on investigators better growth was reported either in Brewer thioglycollate medium (BTM) of a manufacturer or in Fluid thioglycollate medium (FTM) of another manufacturer. Therefore, it was necessary to compare the BTM and FTM from both maufacturers.
Dehydrated FTM (BBL, FTM-B; Difco, FTM-D) and BTM (BBL, BTM-B; Difco, BTMB) were rehydrated and 30-mL amounts were distributed into vials. BACTEC NR 7A medium was used for comparison. Clostridium perfringens, Bacteroides capillosus, B. corporis, B. fragilis, B. thetaiotaomicron, Gemalla morbillorum, Streptococcus intermedius, Peptostreptococcus anaerobius, P, asaccharolyticus, P magnus, P. micros, P. prevotii and p. productus were investigated in this study.
Growth of C. perfringens was rapid in all of the media. Growth of B. capillosus and B. corporis were slow, but similar in all of the media. Better growth of B. fragilis was noted in BTM-D, and B. thetaiotaomicron in FTM-B, respectively. G. morbillorum showed better growth in BTM-D only, and S. intermedius in BTM-D, FTM-B and FTM-D. Better growth ofPeptostreptococcus observed were: P. anaerobius in BTM-D, P. prevotii and P. productus in FTM-B, P. asaccharolyticus, P. magnus and P. micros in BTM-B and BTM-D, respectively. With the exception of P. anaerobius and P. micros, all of the test organisms grew well in NR 7A medium.
In the cooked meat-supplemented FTM-D, the growth of P. anaerobius and P micros were improved and the growth of B. capillosus, P. productus and G. morbillorum became as good as in NR 7A.
It is concluded from this study that none of BTM-B, BTM-D, FTM-B and FTM-D support good growth of all anaerobic bacteria, but in general, growth of the organisms are better in BTM than in FTM and that cooked meat supplementation may improve the performance of various thioglycollate media.
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KEYWORD
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Thioglycollate media, BTM-B, BTM-D, FTM-B, FTM-D
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